Innate immune factor gene expression profiles in patients with atopic dermatitis

Atopic dermatitis is a multifactorial genetically determined inflammatory skin disease characterized by itching, chronically relapsing dermatitis, age-related features of localization and morphology lesions. The pathogenesis atopic complex includes epigenetic alterations, involved in the genomic adaptation, immune response reactions dysfunction epithelial barrier that together trigger development dermatitis. aim this study to detect expression level for IL4, IL13, IL33, TLR2, TLR9 genes biological materials patients. targeted further evaluation were selected according our previous findings on genome-wide methylation study. We detected cascades with differentially methylated are most likely take place Thus, we investigated levels IL4 , skin, peripheral blood mononuclear cells whole using RT-PCR 55 pediatric patients 26 healthy volunteers, 50 adult Statistical analysis was performed use Kruskal-Wallis H test Mann-Whitney U test. Targeted revealed samples 12 times significantly lower (p < 0.0001, p 0.0005) lesional skin; there 6-fold decrease case TLR2 0.01). results differed assessed targets higher before treatment. have also found out those differences strongly pronounced especially an elder age group (12-18 y.o.). Studying IL33 gene adults its moderate form AD. Besides, concluded locally affected may dominate; Th2 apparently takes place. New insights immunological markers links among them shed light pathogenic mechanisms. molecules could play role as potential therapeutic management approach